| overview | acid black ATT is dark brown powder. Soluble in water, the aqueous solution is blue-black, add concentrated hydrochloric acid to produce a green blue precipitate; add sodium hydroxide solution to produce a blue precipitate. Soluble in ethanol, blue, slightly soluble in acetone, insoluble in other organic solvents. It is blue-green in concentrated sulfuric acid, and it produces a dark green blue precipitate after dilution; it is a dark green solution in concentrated nitric acid and then turns into sauce red; it produces a blue precipitate in 10% sodium hydroxide solution. When dyeing, the color of copper ions changes slightly; the color of iron ions is slightly light green. |
| use | the main use of acid black ATT: dyeing of proteins on polyacrylamide gel, agarose gel and nitrocellulose membrane. After electrophoresis, it is recommended to fix the protein on the gel. The glue is dyed with a 7% (v/v) acetic acid solution of 0.1% amino black 10B for at least 2 hours, and then decolorized with a 7% (v/v) acetic acid solution. The detection sensitivity is about 20% of Coomassie Brilliant Blue R250.
mainly used for dyeing wool, silk, nylon and their blended fabrics |